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Clinical Microscopy Quiz 3
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Amady S
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The pH indicators used on the Multistix strip are
methyl red
and
bromothymol blue.
The pH indicator used on Clinistrip is
phenolphthalein.
Urine pH can reach 9.0 if it is has been
sitting for a while
because
urea
is broken down to
ammonia.
Reasons urine could be acidic:
Protein diets
,
Cranberry intake
,
Respiratory acidosis
,
Severe diarrhea
,
Starvation
, UTI (
E. Coli
).
Reasons urine could be alkaline:
Vegetarian Diet
,
Hyperventilation
,
UTI
(pseudomonas, proteus),
Protein reagent runoff
Mucus and casts are made from
Tamm-Horsfall protein
that is secreted by the
tubules.
The protein reagent pad is very sensitive to
albumin.
The glucose reagent pad uses the
glucose oxidase
method.
Glucose Oxidase: Double sequential enzyme reaction
Glucose
+
O2
->In the presence of
glucose oxidase
->
Gluconic Acid
+
H2O2
H2O2
+
Chromogen
->(In the presence of
peroxidase
)->
Oxidized chromogen
+
H2O