Clinical Microscopy Quiz 3

Cards (9)

  • The pH indicators used on the Multistix strip are methyl red and bromothymol blue.
  • The pH indicator used on Clinistrip is phenolphthalein.
  • Urine pH can reach 9.0 if it is has been sitting for a while because urea is broken down to ammonia.
  • Reasons urine could be acidic: Protein diets, Cranberry intake, Respiratory acidosis, Severe diarrhea, Starvation, UTI (E. Coli).
  • Reasons urine could be alkaline: Vegetarian Diet, Hyperventilation, UTI (pseudomonas, proteus), Protein reagent runoff
  • Mucus and casts are made from Tamm-Horsfall protein that is secreted by the tubules.
  • The protein reagent pad is very sensitive to albumin.
  • The glucose reagent pad uses the glucose oxidase method.
  • Glucose Oxidase: Double sequential enzyme reaction
    1. Glucose + O2 ->In the presence of glucose oxidase -> Gluconic Acid +H2O2
    2. H2O2 + Chromogen ->(In the presence of peroxidase)-> Oxidized chromogen + H2O