Substrate Concentration

• Effect of Substrate Concentration

  • At low substrate concentration
  • Excess enzymes
  • Many free active sites
  • Initial steep increase in rate
  • Increasing substrates
  • Increases successful collisions and ESCs
  • Substrate concentration increases further
  • Active sites are all occupied
  • No further rate increase can occur (plateau)
  • Rate is now limited by enzyme concentration
  • Maximum value/velocity (V max) reached
  • Rate remains constant