required practical

• What is the first step in preparing a food sample for tests?

  Break it down using a pestle and mortar
• Why do you add distilled water to the ground food sample?

  To dissolve some of the food
• What should you use to filter the food solution?

  A funnel lined with filter paper
• What is the purpose of the Benedict's test?

  • To test for reducing sugars
  • Involves heating with Benedict's solution
  • Color change indicates sugar presence
• At what temperature should the water bath be set for the Benedict's test?

  75 degrees Celsius
• What color change indicates the presence of reducing sugars in the Benedict's test?

  From blue to green, yellow, or brick-red
• What is the procedure for testing starch using iodine solution?

  1. Prepare a food sample in a test tube
  2. Add a few drops of iodine solution
  3. Gently shake to mix
  4. Color change indicates starch presence
• What color change indicates the presence of starch when using iodine solution?

  From browny-orange to blue-black or black
• What is the purpose of the Biuret test?

  • To test for proteins
  • Involves adding biuret solution
  • Color change indicates protein presence
• What color change indicates the presence of proteins in the Biuret test?

  From blue to purple
• What is the procedure for testing lipids using ethanol?

  1. Add ethanol to the food solution
  2. Observe for cloudiness
  3. Cloudy indicates lipids present
• What does a cloudy solution indicate when testing for lipids?

  That lipids are present
• What will happen to the solution if no lipids are present when using ethanol?

  It will remain colorless