Exercise 4: Photosynthesis

• Photoautotrophic -characteristic of organisms such as plants that produce their own food through photosynthesis
• What is the food for plants in photosynthesis?

  Glucose
• What is glucose stored as in the plant?

  Starch
• Besides water, what does photosynthesis require?

  Carbon dioxide
• What is the color of phenol red in the test tube containing water when using a soda straw to blow?

  Yellow
• What is the acid that turns the phenol red solution yellow?

  Carbonic acid
• What is the color of phenol red when the environment is basic?

  Pink
• What is the relationship of rate of photosynthesis to the brightness of the light?

  Direct
• Cyclosis - Streaming cytoplasm where the chloroplasts move around to optimize light absorption for photosynthesis
• Where does photosynthesis occur in plants?

  Chloroplasts
• Chromatography - A technique used to separate mixtures of substances based on their solubility
• What is the stationary phase of the chromatography set-up?

  Filter paper
• What is the mobile phase of the chromatography set-up?

  petroleum-ether acetone solvent
• What are the respective Rf values for Carotene, chlorophyll a, chlorophyll b and Xanthophyll?

  0.99; 0.30; 0.13; 0.40-0.68
• Among the following pigments, which is the most polar?

  Chlorophyll B
• Among all the pigments, which is the least polar?

  Carotene
• What is the polar component of the filter paper used in the chromatogram?

  Cellulose
• In the image provided, what do A and B represent?

  A: Solvent front line

  B: Spot line

  
• What is the ratio of petroleum-ether in the acetone solvent?

  95:5