Micro Lab Exam 1

• Place to lay the microscope slide while viewing

  stage
• holds the microscope slide in place

  stage clip
• the microscope should be carried by holding onto the

  arm and base
• ocular lens

  10x magnification
• rotates to change between magnifications

  revolving nosepiece
• used to focus light onto specimen and make illumination more uniform

  condenser
• the amount of light is controlled by

  adjusting the rheostat and opening/closing the iris diaphragm
• moves the stage left/right and front/back

  mechanical stage adjustment knob
• moves the stage up/down

  coarse focus knob
• brings the slide into sharp focus

  fine focus knob
• two ocular lenses

  binocular
• two lens systems

  compound
• 4x ocular lens

  scanning
• 10x ocular lens


  low/dry
• 40x ocular lens

  high/dry
• 100x ocular lens

  oil immersion
• ocular lens magnification

  10x
• total magnifications =

  ocular x objective
• 10x x 4x =

  40x
• 10x x 10x =

  100x
• 10x x 40x =

  400x
• 10x x 100x =

  1000x
• microbes are

  ubiquitous in the environment, they can be isolated from many environments
• microbes can be derived from

  • soil
  • water
  • plants
  • animals
  • hot acid pools
• microbes that don't reside on a specific plant or animal host

  free-living
• are not known to cause disease

  nonpathogenic
• known to cause disease

  pathogenic
• decompose organic matter

  saprophytes
• microbes benefit the host

  mutualistic
• microbe benefits, but has no significant effect on the host

  commensalism
• capable of producing a disease state if introduced into a suitable part of the body

  opportunistic pathogens
• any area where a microbe residers and serves as a potential source of infection is called

  reservoir
• used to grow microbes when fresh cultures or large numbers of cells are required

  broth
• primarily for growth and maintenance of stock cultures

  slant
• aid in determining oxygen requirements and motility

  deep
• used for isolating individual species from a mixed culture

  plate
• when inoculating a slant, both, or plate use

  the inoculation loop
• when inoculating a deep use

  the inoculating needle
• Wet mount and hanging from slides

  • allow observation of living, motile, unstained organisms
  • helps determine motility and morphology of bacteria
  • provides a sense of oxygen requirements
• What is the advantage of using a Hanging drop slide

  it doesn't dry out as quickly as a wet mount