Bacterial Smear and Preparation

Cards (55)

  • type of slide needed for bacterial smear and staining
    frosted
  • how to sterilize the frosted slide
    tissue and pass through heat
  • your culture is always placed ...
    upside down
  • why culture is placed upside down?
    to avoid moisture that will grow more bacteria
  • after getting your colony, what solvent will you mix it with?
    1 drop of NSS solvent
  • ideal size of the smear
    1 inch by 1 and a half inch or thumb size
  • what will you do before and after opening your culture?
    heat
  • after air drying what will you do?
    heat fix
  • in broth, how will you remove the cap?
    using dominant hands pinky finger
  • to know if the broth has bacteria, it must be
    turbid
  • no floating substances or solid substances that can be seen in the solution/ smear
    homogenous
  • concentration is
    uniform
  • Good smear is for:
    • Morphology
    • Arrangement
    • Constituents (cell components)
  • one cocci

    coccus
  • two cocci
    diplococci
  • 4 cube cocci
    tetrads
  • 8 cocci (rubics cube)

    sarcina
  • 10 grape like 

    staphylococcus
  • chain lime cocci
    streptococci
  • cocci commonly found in neisseria spp.
    diplococci
  • cocci commonly found in micrococci
    tetrads
  • cocci commonly found is micrococcus lukeus
    sarcina
  • commonly found in staphylococcus sp.
    clusters/ staphylococci
  • commonly found in streptococcus
    chain/ streptococci
  • combination of cocci and bacilli
    coccobacillus
  • one bacilli
    bacillus
  • two side by side bacilli
    diplobacilli
  • chain bacilli
    streptobacilli
  • bacilli in fence like structure
    Palisades
  • primary stain in gram staining
    violet for 1 minute
  • essential in gram staining process because it holds down molecules of a staining onto microorganism
    mordant - grams iodine - 1 minute
  • used to hasten the process of holding down the stain of the cell components onto a microorganism (not essential)
    accentuator
  • acetone alcohol or ethanol alcohol
    decolorizer
  • used to stain the gram negative components of the components of the specimen
    Counterstain/Secondary stain - Safranin - 1 minute
  • GS - Gram (+)
    violet
  • GS- Gram (-)
    pink
  • also called as hot method in acid fast staining
    ziehl neelsen method
  • AFS bacteria are gram positive because of its 

    thick peptidoglycan
  • primary stain

    carbol fuchsin
  • Mordant of AFS
    heat