Compound Microscope

• Optical instrument that is used to observe tiny objects

  Microscope
• First person to see live bacteria (animalcules) and protozoa. Father of:

  • Microbiology
  • Bacteriology
  • Protozoology

  Anton Van leeuwenhoek
• Used to observe morphology of microorganisms such as bacteriabacteria, protozoa, fungi, and algae (stained/unstained)

  Brightfield Microscope
• Unstained organism observed against dark background; usefuluseful for examining thin spirochetes

  Darkfield Microscope
• Can be used to observe unstained living microorganisms

  Phase Contrast Microscope
• Fluorescent dye attached to organism; primarily an immunodiagnostic technique

  Fluorescence Microscope
• Base, Arm, Stage, Substage, Mechanical stage

  Mechanical system
• Nose piece, Objective, Eyepiece, Focal length

  Lens system
• On/Off switch, Fine and course adjustment knob, Iris diaphragm, Condenser, light source

  Other parts
• Holds the objective lenses

  Revolving nosepiece
• Used to magnify objects


  Objective lenses
• Flat surface on which the specimen is placed

  Stage
• Used to move the stage and microscope slide

  Stage adjustment knob
• Used to adjust amount of light passing through condenser

  Iris diaphragm
• Contains a lens system that focuses light onto the specimen or slide

  Condenser
• Controls the amount of light emitted from light source

  Rheostat control knob
• Used to adjust the amount of light passing through the collector lens

  Field diaphragm lever
• Contains the light source

  Base
• Used to adjust height of the condenser

  Condenser control knob
• Holds the ocular lenses in their properproper location

  Binocular body