Cb1a

• Magnification in microscopy:

  • Magnification is achieved through the combination of the eyepiece and objective lenses
  • The total magnification is calculated by multiplying the magnification of the eyepiece by the magnification of the objective lens
• Robert Hooke discovered cells in 1665 using a microscope
• Resolution in microscopy:

  • The resolution of a microscope determines the level of detail that can be observed
  • Smaller wavelengths of light or electrons produce higher resolution images
• Standard forms of measurement in microscopy:

  • Millimeters (mm)
  • Micrometers (µm)
  • Nanometers (nm)
  • Picometers (pm)
• Electron microscopes are more powerful than light microscopes, providing higher resolution images
• The nucleus, chromosomes, and other cellular activities inside the cell can be observed using a microscope
• The size of objects in a microscope is measured in actual size and magnification is used to observe objects that are smaller than the resolution limit of the human eye
• Microscope parts:

  • Arm
  • Base
  • Stage
  • Condenser
  • Objective lens
  • Eyepiece
  • Adjustment knob