Replication of Dna

• What is Cell Division called?

  Mitosis
• Why do the Cells get the same number of Genes?


  So they are Identical
• DNA replication is ____

  Semi-Conservative
• Step 1 - they ____ unwinds, separating the two strands?

  DNA double helix
• A primer binds to what side?

  3' end
• DNA ____ adds nucleotides to the DNA strand.

  Polymerase
• What does DNA polymerase need to start replication?

  Primers
• Leading strand is replicated __

  Continously
• Lagging strand is replicated in __

  Fragments
• What is PCR used for?

  to amplifying DNA
• What temperature is used to denature DNA?

  92-98 (95) degrees
• What temperature is PCR lowered to for the primers to anneal?

  50-65 (55) degrees
• What temperature is used to add nucleotides to the DNA strand?

  70-80 (75) degrees