Effect of pH and temperature on enzymes

• A number of factors affect the activity of enzymes. The rate of activity may be increased by raising the concentration of the enzyme or the substrate. Two other factors that affect enzymes are temperature and pH
• All enzymes have an optimum temperature which they work best. Enzymes in the human body have evolved to work best at our body temperature. (37 degrees C)
• Like any reaction, increasing the temperature increases the rate (at first).
• When increasing temperature, the enzymes and substrate have more kinetic energy, they move faster, and are more likely to collide to form enzyme-substrate-complexes.
• If the temperature gets too hot, some of the bonds holding the nezymes together break. This changes shape of the active site and the enzyme is denatured.
• The pH also affects the enzymes, if its too high or too low, the bonds holding the enzyme together break, changing the shape of the active site and the enzyme denatures
• All enzymes have an optimum pH that they work best at, usually it's pH 7, but not always.