Vitamin C content of food and drink

• What reagent is used to test for Vitamin C, and what is observed?

  • DCPIP

  • Colour changes from blue to colourless
• Outline the procedure to use DCPIP to find the vitamin C content of different samples.

  • Transfer 1cm of DCPIP solution into a test tube with a pipette.

  • Add Vitamin C solution dropwise to the DCPIP solution. Shake after each drop.

  • Record the volume of Vitamin C that is required to change the colour of the DCPIP.

  • Repeat the experiment and replace the Vitamin C solution with the fruit juices to be tested.
• State a hazard and safety precaution involved in this practical

  • DCPIP is an irritant, wear eye protection and avoid contact with skin