Growth

• What is the aim of the practical investigation?

  To investigate the effect of antiseptics or antibiotics
• What method is used to measure the effect of antiseptics or antibiotics?

  Measuring zones of inhibition on agar plates
• How should filter paper discs be treated before placing them on agar plates?

  Soaked in different antiseptics or antibiotics
• What is the significance of measuring the zone of inhibition?

  To compare the effectiveness of different substances
• What should be provided for the practical investigation?

  Prepared Petri dish plates
• Why is preventing contamination important in microbiology investigations?

  To ensure only the effect of antiseptics or antibiotics is studied
• What is the ideal temperature for bacterial growth in this practical?

  25 °C
• Why is it important to maintain a temperature of 25 °C?

  To reduce the chances of growing harmful pathogens
• Which bacterial cultures are most likely used in this practical?

  Safe strains of coli or Micrococcus luteus
• What are the steps involved in the practical investigation of antiseptics or antibiotics?

  1. Use aseptic technique to prepare agar plates
  2. Soak filter paper discs in antiseptics/antibiotics
  3. Place discs on agar plates with bacteria
  4. Measure zones of inhibition
  5. Calculate the area of each zone