enzymes

• What is the aim of the enzyme experiment?

  To investigate the effect of pH on amylase
• What enzyme is used in this experiment?

  Amylase
• What substrate does amylase act upon?

  Starch
• What product is formed when amylase breaks down starch?

  Maltose
• What indicator is used to test for starch presence?

  Iodine solution
• What colour change indicates starch presence with iodine?

  Blue-black
• What colour indicates that starch is no longer present?

  Orange-brown
• What technique is used to monitor the reaction progress?

  Continuous sampling technique
• What is the purpose of the pH buffer solution in the method?

  To maintain a specific pH for the reaction
• What is the independent variable in this experiment?

  pH
• What is the dependent variable in this experiment?

  The rate of reaction of amylase
• How can temperature be controlled in this experiment?

  By using a water bath at constant temperature
• Why is it important to control temperature in this experiment?

  Temperature affects enzyme activity
• How could a colorimeter improve the experiment's accuracy?

  It quantitatively measures colour change
• What does a faster rate of reaction indicate about enzyme activity at a pH?

  Enzyme is working more effectively at that pH