micp chap 7
Cards (55)
- use of chemical agents on living tissue to prevent the spread of microorganism either inhibiting their growth or destroy them
- an agent, physical or chemical, capable of inhibiting the growth of bacteria without necessarily killing them
- an agent, physical or chemical, that kills bacteria
- process by which most microbial forms on inanimate objects are killed without necessarily destroying saprophytes and bacterial endospores, which leads to reduction in the number to a level tehy cant produce infection
- capable of destroying spores, fungi, and viruses
- killing all microbial forms, including spores
- most common physical method of sterilization
- rate of killing is expressed in
- moist heat has greater killing action than dry heat
- preferred over dry heat because of its more rapid killing action
- destroying disease-causing organisms in milk and milk products as well as other beverages
- destroy contaminating bacteria in vaccine preparations
- inactive bacteria contaminating serum prep and is done by heating at 56 degree C for several succesive days
- used to solidify and disinfect egg containing and serum containing media
- pasteurization, vaccine bath, serum bath, inspissation
- boiling, tyndallization,
- autoclave
- utilizing water at boiling temp of 100 degree C
- aka intermittent sterilization and involves exposing the material to be sterilized to live steam at 100 degree C for 30 to 90 mins for 3 days
- most efficient method of sterilization bcs it can destroy all microbial forms
- used to sterilize in enclosed tubes, oils, jellies, powders, and glasswares such as test tubes and petri dishes
- ised to sterilize articles like bacteriological wire loops, straight wires, tips of forceps, and searing spatulas
- makes use of the bunsen burner or alcohol lamp
- aimed at burning organism into ashes
- articles are placed in the oven with a temp of 160 C for one hour
- articles to be sterilized are placed in a conveyor belt and passed thru a tunnel that is heated by infrared radiators
- based on the principle of depriving the microorganism of moisture
- not reliable method of sterilization bcs most pathogenic organisms are resistant to low temps
- mechanical sieving that does not kill microorganisms but merely seperates them from the fluid
- inducing formation of thymine-thymine dimers, resulting in lethal framshift mutations
- causing formation of free radicals that chemically interact with proteins and nucleic acids, resulting in cell death
- electron beams and electromagnetic rays (gamma rays)
- produce from nuclear disintegration of selective radioactive isotopes
- some bacteria can be killed after exposure to certain frequency of sound waves
- when the concentration of the fluid surrounding the organism is altered, this will cause bacterial cell to collapse
- compounds have long chain hydrocarbons that are fat soluble and charged ions that are water soluble
- detergents where fat soluble portion is positively charged due to combination with quarternary nitrogen atom