GMO in bacteria

• Describe the steps to make a GMO bacteria

  1. Identified desired gene is taken from a cell
  2. Amplified via PCR with DNA polymerase
  3. Isolated with RE
  4. ALSO, cut plasmids from bacteria with RE
  5. Leaves sticky ends
  6. Plasmid & desired gene spliced, forms phosphodiester bonds using DNA ligase
  7. Recombinant plasmid produced
  8. Inserted into bacteria
  9. Multiply
  10. Desired gene transcribed & translated into proteins in large quantities
  11. Extracted from culture
  12. Sterilised