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BIOLOGY
REQUIRED PRACTICALS
7 - CHROMATOGRAPHY
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Created by
Jasmine Singh
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Cards (8)
AIM?
chromatography
to investigate
pigments
isolated from
leaves
of different plants
WHAT DO RATES OF MIGRATION DEPEND ON?
solubility
,
mass
and
affinity
to
paper
(Rf:
0
- not attracted to
mobile phase
,
1
- not attracted to
stationary phase
)
KEY PRACTICAL POINTS?
acetone
used to
grind leaves
into
solution
of
leaf pigment
applied using a
capillary tube
-> not same as
solvent
(
ethanol
/
water
)
IV?
type
of
leaf
DV?
Rf value
(
type
of
chlorophyll
)
CVs?
volume
of
acetone
type
of
paper
type
of
solvent
volume
of
solvent
RISKS?
leaf
is a
biohazard
,
bacteria
solvent
/
acetone
are
flammable
IMPORTANT FINAL STEP OF PRACTICAL?
compare
Rf
to
database
to
identify pigment
(ensure
paper
, same
solvent
)
