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BIOLOGY
REQUIRED PRACTICALS
9 - RESPIRATION
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Created by
Jasmine Singh
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Cards (9)
AIM?
effect of named
variable
on rate of
respiration
of
cultures
of
single-celled organisms
WHY DOES THIS EXPERIMENT WORK?
instead of using
electrons
in
respiration
to produce
ATP
, they will be
accepted
by
methylene blue
(turns from
blue
to
colourless
)
KEY PRACTICAL POINTS?
colorimeter
glucose solution
RATE OF REACTION?
1
/
time
IV?
temperature
/
pH
DV?
time taken
for
methylene blue
to
decolourise
(
rate
=
1 / time
)
CVs?
yeast species
yeast mass
volume
of
glucose solution
presence
of
oxygen
volume
of
methylene blue
concentration
of
methylene blue
RISK?
methylene blue
is an
irritant
KEY POINTS ABOUT USING A POTOMETER?
cut underwater
set up apparatus underwater
air tight
turgid shoots
