11 - GLUCOSE CONCENTRATION

• AIM?

  produce dilution series of glucose solution and use colorimeter to produce calibration curve to identify concentration of glucose in unknown sample ('urine')
• WHAT SHOULD BE USED IN THIS EXPERIMENT?

  quantitative Benedict's to create calibration curve using known concentrations

  -> reduces Cu (2+) to Cu (+) to produce Cu2O
• IV?

  concentration of glucose in sample
• DV?

  absorbance (colorimeter)
• CVs?

  • concentration of Benedict's
  • volume of sample
  • time for Benedict's
• WHAT IS A NORMAL URINE GLUCOSE CONCENTRATION?

  0 to 0.8 mmoldm^-3
• RISK?

  Benedict's is an irritant so wear eye protection