MICROBIOLOGY AND PARASITOLOGY LAB
Subdecks (2)
Cards (60)
- Method of staining easy to visualize the cell shape, size and arrangement of bacteria.
- Safranin, Methylene blue or crystal violet
- distinguish gram-positive bacteria from gram-negative.
- Gram staining (gram-positive) Color
- Gram staining (gram-negative) Color
- All are gram-positive. (Gram Staining)
- all are gram-negative (Gram-staining)
- Gram staining Primary Stain
- Gram staining Mordant
- Gram staining Decolorizer
- Gram staining counterstain or secondary stain
- stain used for bacteria with high lipid content in their cell wall.
- Known as the “Hot Method” because it requires steam-bathing the prepared smear after addition of the primary dye.
- Known as the “Cold Method” as it does utilize heat after addition of the primary stain, which is oil-based.
- Ziehl-Neelsen stain Primary stain
- Ziehl-Neelsen stain Decolorizer
- Ziehl-Neelsen stain secondary stain
- Kinyoun stain Primary stain
- Kinyoun stain Decolorizer
- Kinyoun stain Secondary Stain
- Acellular organisms
- Possess only single nucleic acid, either DNA or RNA but never both.
- Special type of viruses primarily infect bacteria
- Are prokaryotic cells with majority having an outer covering called the cell wall that is composed mainly of peptidoglycan.
- Eukaryotic cells with an outer surface composed mainly of chitin
- Representatives for parasites
- Plant-like organisms whose outer surface consist primarily of cellulose
- Major surface antigens of gram-positive organisms
- Molecules include neutral sugars
- Bi-layered structure where inner leaflet is composed of a lipopolysaccharides (LPS).