rDNA Tools

• Mutagen


  physical and chemical change agents that produce mutations

  used to create changes in microbes genomes to change phenotypes
• Restriction Sites


  DNA sequences are palindromes

  specific sequences of bases that guides Restriction Enzymes bind, recognize and cut to
• Ligase


  Glues DNA fragments together
• Vectors


  vehicle that transports the gene into a host cell, which is usually a bacterium although other types of living cells can be used

  example of vectors is plasmid
• Transformation


  process of inserting foreign genes into a bacteria
• Antibiotic Selection


  identities recombinant bacteriabor which bacteria took the rDNA
• Restriction Enzymes


  aka Endonuclease bacterial enzymes that cut DNA only at restriction sites the enzymes must be the same when cutting for the gene and plasmid