W5L10 - Cloning MCQ

• In vivo means:

  1. Taking place in a dead cell or organism.
  2. Taking place in a living cell or organism.
  3. Taking place in test tube, flask or petri-dish (a container) outside a living cell or organism.

  2
• In vitro means:

  1. Taking place in a dead cell or organism.
  2. Taking place in a living cell or organism.
  3. Taking place in test tube, flask or petri-dish (a container) outside a living cell or organism.


  3
• The purpose of PCR is to:

  1. rapidly amplify DNA at a linear rate
  2. rapidly amplify DNA at a logarithmic rate
  3. rapidly amplify DNA at an exponential rate
  4. rapidly amplify RNA at a linear rate
  5. rapidly amplify RNA at a logarithmic rate
  6. rapidly amplify RNA at an exponential rate

  3
• In a typical PCR reaction you will have:

  1. DNA polymerase (Taq); PCR Primers; Template DNA; dNTPs (dATP, dCTP, dGTP) and a reaction buffer.
  2. DNA polymerase (Taq); PCR Primers; Template DNA; dNTPs (dATP, dCTP, dGTP, dTTP) and a reaction buffer.
  3. DNA polymerase (Taq); PCR Primers; dNTPs (dATP, dCTP, dGTP, dTTP) and a reaction buffer.
  4. RNA polymerase (Taq); PCR Primers; Template RNA, dNTPs (dATP, dCTP, dGTP, dTTP) and a reaction buffer.
  5. RNA polymerase (Taq); PCR Primers; Template RNA; dNTPs (dATP, dCTP, dGTP, dTTP) and a reaction buffer.

  2
• An endonuclease will...

  1. always cut at a specific defined site within the DNA molecule and all endonuclease only produce blunt-ended cuts.
  2. always cut at a specific defined site within the DNA molecule and produce either blunt or sticky DNA ends depending on the nuclease
  3. cut at random sites in within the DNA molecule and produce either blunt or sticky DNA ends depending on the nuclease.
  4. only remove the nucleotide at the 3-prime end of the DNA molecule.
  5. only remove the nucleotide at the 5-prime end of the DNA molecule.

  2
• Which of the following completes this sentence? Cutting the plasmid, and the DNA to be inserted, with two different restriction enzymes is better than cutting with one because cutting with two helps to...

  1. get the correct orientation of the insert in the plasmid.
  2. prevent the insert from being incorrectly orientated, but has no impact on the ligation of the plasmid with itself.
  3. prevent the plasmid from re-ligating on itself.
  4. the plasmid from re-ligating on itself, it does not prevent the insert from being inserted in the incorrect orientation.

  1 and 3
• In the blue-white selection procedure, which of the following are true?

  1. All the blue colonies on the plate contain the plasmid and the plasmid does not contain the insert.
  2. All the blue colonies on the plate contain the plasmid, and the plasmid contains the insert.
  3. All the colonies on the plate contain the plasmid.
  4. All the white colonies on the plate contain the plasmid and the plasmid contains the insert.
  5. All the white colonies on the plate contain the plasmid, and the plasmid does not contain the insert.
  6. None of the above are correct.

  1, 3, 4
• In which order are the steps in a PCR reaction?

  1. Anneal -> Denature -> Extend
  2. Denature -> Anneal -> Extend
  3. Denature -> Extend -> Anneal
  4. Extend -> Anneal -> Denature
  5. None of the above are correct

  2
• Taq polymerase was first isolated from a bacteria growing...

  1. in a dark, cold cave.
  2. in a hot spring.
  3. on a banana.
  4. on an iceberg.
  5. on melon.

  2