W6L11 - Proteomics MCQ

• Two-dimensional gel electrophoresis (2DE) is:

  1. a column based method which separates proteins based on their charge at a given pH and then their molecular weight
  2. a column-based method which separates proteins based on their 3-D structure and molecular weight
  3. a gel-based method which separates proteins based on isoelectric focusing point and molecular weight
  4. a gel-based method which separates proteins based on their 3-D structure and charge
  5. a waste of time

  3
• Multi-dimensional liquid chromatography (MDLC) is:

  1. a column based method which uses two different columns that separate proteins based on two different physical characteristics (e.g. molecular weight and charge)
  2. a gel-based method which separates proteins based on isoelectric focusing point and molecular weight
  3. a gel-based method which separates proteins based on their 3-D structure and charge
  4. a waste of time

  1
• Microarrays look for...

  1. Changes in the DNA levels
  2. Changes in the mRNA levels
  3. Changes in the protein levels
  4. Changes in the rRNA levels
  5. Changes in the tRNA levels
  6. Changes in the transcriptome

  2 and 6
• Match the answer to the question

  Proteomics is 3

  Transcriptomics is 4

  Metabolomics is 2

  Genomics is 1