R.P microscopy

• Magnification =

  Image size ÷ actual object size
• Why do we stain things?

  to make things visible e.g cell wall, nucleus
• Total magnification= 


  magnification of eye peice lens x magnification of objective lens
• Size of real object =

  Image size ÷ magnification
• Microscopy


  The use of microscopes
• How light microscopes work

  1. Light from the room hits the mirror

  2. Light reflected upwards through the object

  3. Light passes through the objective lens

  4. Light passes through the eyepiece lens

  5. Light enters the eye
• Object


  The real object or sample that you're looking at
• Image


  The image that we see when we look down the microscope
• Magnification


  How many times larger the image is than the object
• Magnification = image size / object size
• Resolution


  The shortest distance between two points on an object that can still be distinguished as two separate entities
• Higher resolution


  More details can be seen, less blurry the image
• Light microscopes


  Microscopes that use light, small, easy to use, relatively cheap
• Resolution of light microscopes


  Limited to 0.2 micrometers, any details less than 0.2 micrometers apart will appear blurry
• What light microscopes can be used to see


  • Individual cells like onion cells
• Electron microscopes


  Really big, very expensive, hard to use, only used by scientists in laboratories