Pt 2

• -no clearly defined


  Acid Phosphatase ACP
• -prostatic specific antigen


  prostatic adenocarcinoma
• E.C. 1.1.1.27


  Lactate Dehydrogenase (LD/LDH)
• -rape, 4 days vaginal swab


  forensic clinical chemistry
• E.C. 1.1.1.49


  Glucose-6-Phosphate Dehydrogenase (G6PD)
• E.C. 1.16.3.1


  Ceruloplasmin/Ferroxidase
• -bone metastases

  -osteoclasts

  -idiopathic thrombacytopenic purpura

  Paget’s disease
• -source of increase ALP

  -liver marker


  Tissue sources
• E.C. 2.7.3.2 


  Creatine Kinase (CK)/ATP-CREATINE-N-PHOSPHOTRANSFERASE/ CREATINE PHOSPHOKINASE
• -catalyzes the breakdown of starch and glycogen

  AMYLASE (AMS/AMY)
• -integral part of the enzyme

  Calcium
• -activation


  Calcium and chloride
• E.C. 2.6.1.1


  ASPARTATE AMINOTRANSFERASE(AST) / SERUM GLUTAMIC-OXALOACETIC TRANSAMINASE (SGOT/GOT)
• Acinar cells of the pancreas

  P-P1, P2, P3
• E.C. 2.6.1.2


  ALANINE AMINOTRANSFERASE (ALT)/ SERUM GLUTAMIC-PYRUVIC TRANSAMINASE
• Salivary glands


  S-S1,S2,S3
• E.C. 2.3.2.2


  GAMMA-GLUTAMYLTRANSFERASE (GGT)/ GAMMA GLUTAMYL TRANSAMINASE PEPTIDASE
• E.C. 2.1.3.3


  ORNITHINE CARBAMOYL TRANSFERASE (OCT)
• E.C. 3.1.3.1


  ALKALINE PHOSPHATASE (ALP)/ ORTHOPHOSPORIC MONOESTER PHOSPHOHYDROLASE/ ALKALINE OPTIMUM
• E.C. 3.1.3.2


  ACID PHOSPHATASE (ACP)/ ORTHOPHOSPHORIC MONOESTER PHOSPHOHYDROLASE/ ACID OPTIMUM
• E.C. 3.1.3.5


  5' NUCLEOTIDASE (5'NT)
• E.C. 3.2.1.1


  AMYLASE (AMS/AMY)/ ALPHA-1,4 GLUCAN-4-GLUCOHYDROLASE
• • rises 5-8 hours
  • peak at 24hours
  • elevated within 2-3days
  • normalize within 3-5days

  Acute Pancreatitis
• -parotits

  Salivary Gland Diseases
• Ectopic pregnancy
• E.C. 3.1.1.3

  LIPASE (LPS) / TRIACYLGLYCEROL ACYLHYDROLASE
• renal failure but no AP
• E.C. 3.1.1.8


  PSEUDOCHOLINESTRASE (PChE)
• -neoplasm

  -macroamylasemia


  Hyperamylasemia
• E.C. 3.4.15.1


  ANGIOTENSIN-CONVERTING ENZYME (ACE)/PEPTIDYLDIPEPTIDASE A/KINASE II
• E.C. 4.1.2.13


  ALDOLASE (ALD)/FRUCTOSE-1,6-DIPHOSPHATE ALDOLASE
• -dietary TAG

  -2-monoglyceride

  -quinine inhibit lipase activity

  Lipase (LPS)
• -pancreatic tissue and secretions


  L-L1,L2,L3
• LD-1: Acute Myocardial Infarction (AMI)

  LD-2

  LD-3

  LD-4

  LD-5: nonspecific

  LD-6: alcohol dehydrogenase

  LD-X: Abnormal constituent in semen

  LDH Isoenzymes
• -RBC cholinesterase

  -Acetylcholine

  -true cholinesterase

  -rapid termination

  Acetylcholinesterase
• -Newborn screening (G6PD deficiency)

  -megaloblastic anemia

  G6PD
• pernicious megaloblastic anemia

  LDH
• -serum cholinesterase

  -Acylcholine acylhydrolase

  -butyrylcholinesterase

  -acylcholine + water

  -butyrylcholine

  -benzoylcholine

  -serum, liver, heart, pancreas white matter of the brain

  -unknown normal function drugs

  Pseudocholinesterase
• -inceticides

  Ache
• AMI *sensitive indicator

  CK