Laboratory Evaluation of Hemostasis

• useful screening procedure for the extrinsic coagulation

  prothrombin time
• needed for warfarin therapy monitoring

  Prothrombin time
• procedure for prothrombin time:

  PPP + PT reagent (Thromboplastin + CaCl2)
• NV for Prothrombin Time:

  10-12 s
• Most useful procedure for routine screening of coagulation disorders in the intrinsic pathway. For detecting the presence of circulating anticoagulants (inhibitors). and monitoring heparin therapy.

  Activated Partial Thromboplastin Time
• Activated Partial Thromboplastin Time procedure:

  Phospholipid + Activators +CaCl2
• activators in APTT:

  Kaolin

  Celite

  Ellagic

  Micronized Silica
• normal value of APTT

  20-45s
• Used to detect deficiencies in prothrombin, fibrinogen and factor V and X

  Stypven Time
• Stypven Time nv

  6-10 s
• Stypven Time powerful anticoagulant

  Russel's viper venom
• Measures the availability of functional fibrinogen.

  Thrombin Time
• Thrombin Time Normal volume:

  10-14 s
• affected by heparin contamination

  Thrombin Time
• Helpful in testing for functional fibrinogen when the thrombin time is prolonged because of heparin.

  Reptilase time
• venom used in Reptilase time

  Bothrops Atrox snake venom
• Bothrops Atrox snake venom has a _ like activity

  thrombin
• Factor XIII deficiency screening test

  5M urea solubility test
• helpful for the detection of lupus anticoagulant

  DRVVT