Aseptic technique (P6)

• What is the aim of this practical

  To investigate the effect of antimicrobial substances on microbial growth
• what is a zone of inhibition

  a clear area around an antimicrobial disc where bacteria have not grown
• how do you calculate the area of the zone of inhibition

  use the formula for the area of a circle (pi X radius squared)
• name three aseptic techniques used in the experiment

  flaming the loop, working near a Bunsen burner and minimising time containers are open