Microscopes

• What is magnification?

  • How many times larger the image is compared to the object "I AM" triangle
• What is resolution?

  • The ability to determine between 2 seperate points that are close together
• What are the properties of an optical (light) microscope?

  • Condensed beam of light
  • Poor resolution due to long wavelength light
  • Lower magnification
  • Colored images
  • Can view living samples
  • Can view SOME organelles
• What are the properties of an transmission electron microscope (TEM)?

  • 2D image
  • Sample dead in
  • High resolution due to electrons have short wavelengths
  • High magnification
  • Shows internal structures
  • Sample must be ultra thin for electrons pass through the sample
• What are the properties of an scanning electron microscope (SEM)?

  • 3
  • Beam of condensed electrons that bounce off the sample
  • Lower resolution than TEM
  • High magnification
  • Shows surface structures
  • Can be a thicker sample
  • Sample dead in vacuum
  • Black and white image