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Paper 2
rp
Chromatography
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Created by
rej ✯
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Cards (20)
What is the sample of food coloring referred to in the experiment?
U for
unknown
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How many known food colorings are used in the experiment?
Four
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What is the first step in carrying out paper chromatography?
Draw a
horizontal
pencil line
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How far from the bottom should the pencil line be drawn on the chromatography paper?
About 2
cm
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What should be the spacing between the pencil spots on the chromatography paper?
Equal spaces with
1 cm
clear on each side
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What is used to apply the food colorings onto the paper?
A
capillary tube
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Why is it important to keep the spots small on the chromatography paper?
To prevent
colors
from spreading into each other
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What is the depth of water used in the beaker?
1 cm
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What role does the water play in the chromatography process?
It acts as the
solvent
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What must be ensured about the pencil line during the chromatography process?
It must be above the
water surface
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Why should the sides of the paper not touch the beaker walls?
It
interferes
with
water movement
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What is done to reduce evaporation of the solvent during chromatography?
Put a lid on the beaker
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When should the chromatography paper be removed from the beaker?
When the water has traveled
3/4
up
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What should be marked on the paper after removing it from the beaker?
The point where the
water
reached
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What does it indicate if the unknown color separates into three spots?
It is a mixture of three
colors
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How can the identity of the unknown color be determined?
By calculating the
RF values
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How is the RF value calculated?
Distance moved by
chemical
/ distance moved by
solvent
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What does an RF value indicate?
The identity of the
chemical
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What should be done if multiple chemicals have the same RF value?
Repeat the experiment with a different
solvent
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What are the key points to remember during the chromatography process?
Pencil line must be above water.
Sides of paper must not touch beaker walls.
Cover beaker to minimize
solvent
evaporation
.
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