Chromatography

Cards (20)

  • What is the sample of food coloring referred to in the experiment?
    U for unknown
  • How many known food colorings are used in the experiment?
    Four
  • What is the first step in carrying out paper chromatography?
    Draw a horizontal pencil line
  • How far from the bottom should the pencil line be drawn on the chromatography paper?
    About 2 cm
  • What should be the spacing between the pencil spots on the chromatography paper?
    Equal spaces with 1 cm clear on each side
  • What is used to apply the food colorings onto the paper?
    A capillary tube
  • Why is it important to keep the spots small on the chromatography paper?
    To prevent colors from spreading into each other
  • What is the depth of water used in the beaker?
    1 cm
  • What role does the water play in the chromatography process?
    It acts as the solvent
  • What must be ensured about the pencil line during the chromatography process?
    It must be above the water surface
  • Why should the sides of the paper not touch the beaker walls?
    It interferes with water movement
  • What is done to reduce evaporation of the solvent during chromatography?
    Put a lid on the beaker
  • When should the chromatography paper be removed from the beaker?
    When the water has traveled 3/4 up
  • What should be marked on the paper after removing it from the beaker?
    The point where the water reached
  • What does it indicate if the unknown color separates into three spots?
    It is a mixture of three colors
  • How can the identity of the unknown color be determined?
    By calculating the RF values
  • How is the RF value calculated?
    Distance moved by chemical / distance moved by solvent
  • What does an RF value indicate?
    The identity of the chemical
  • What should be done if multiple chemicals have the same RF value?
    Repeat the experiment with a different solvent
  • What are the key points to remember during the chromatography process?
    • Pencil line must be above water.
    • Sides of paper must not touch beaker walls.
    • Cover beaker to minimize solvent evaporation.