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Day 2
Biotechnology
Genetic Engineering
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Created by
Crista Niña Bayaua
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Cards (9)
Genetic Engineering
Manipulating
genetic material
(
DNA
) to achieve the desired goal in a pre-determined way
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Polymerase Chain Reaction
(PCR)
Multiplies the DNA exponentially for
25
to
75
cycles
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Polymerase Chain Reaction (PCR)
1.
Cycle
takes only a
minute
2. Each new segment of
DNA
that is made can serve as a
template
for new ones
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Kary Mullis
Developed
PCR
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Components of PCR
TAQ
Polymerase
(Thermus Aquaticus Polymerase)
Primers
Nucleotides
Thermal
Cycler
PCR
Tube
Temperature
DNA
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TAQ Polymerase
Heat-stable DNA polymerase
extracted from the thermophilic bacteria
Thermus aquaticus
Distributes the replicated DNA's
bases
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Thermal
Cycler
Instrument used in
PCR
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PCR Tube
Small volume reduces the use of expensive
reagents
and allows rapid and efficient heating and cooling in a
thermocycler
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Steps in PCR
1.
Denaturation
(96 Celsius, Separating)
2.
Annealing
(55-65 Celsius, Distributing new bases)
3.
Extension
(72 Celsius, Part 2 of Annealing)
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