Michaelis Menten basics

• Km = k-1 + k2 / k1 = [s] at 1/2 Vmax
• k2 = kcat which is the slow step ES ⇌ E + P unless k3 exists which is EP ⇌ E+P
• first order = k = s⁻¹
• zero order = k = M/s
• second order = k = (M*s)⁻¹
• Keq = k1 / k-1
• V0 = Vmax [S]/ Km + [S] = d[P]/dt
• Vmax = k2[ES]