Enzymatic reactions

• Practical PT1

  1. put a drop of iodine solution into every well in the spotting tile
  2. place a bunsen burner on a heart proof mat and a tripod and gauze over the bunsen burner. Put a beaker on top and heat until its 35 degrees
  3. use a syringe to add 1cm of amylase solution and 1cm of a buffer solution with a pH of 5 to a boiling tube
• Practical PT1

  1. put a drop of iodine solution into every well in the spotting tile
  2. place a bunsen burner on a heart proof mat and a tripod and gauze over the bunsen burner. Put a beaker on top and heat until its 35 degrees
  3. use a syringe to add 1cm of amylase solution and 1cm of a buffer solution with a pH of 5 to a boiling tube, put the tube into the beaker of water for 5 minutes
  4. use a different syringe you add 5cm of starch solution to the boiling tube
• Practical PT2

  5. mix the contents of the boiling tube and start a stopwatch

  use a dropping pipette to take a fresh sample from the boiling tube every 30 seconds and drop it into a well to record how long it takes for the amylase to break down the starch

  6. when the iodine solution remains browny/ orange, start is no longer present. repeat the experiment with buffer solutions with a different PH to see how different pH affects the time taken for the starch to be broken down
• to calculate the reaction use the formula -

  100/time = rate