staphylococcus spp.

• In what family does Staphylococcus spp. belongs?

  Family of: Staphylococcaceae
• The microscopic examination (morphology) of Staphylococcus spp. is:

  • Cocci in singly
  • Cocci in pairs
  • Cocci in grape-like clusters
• Staphylococcus spp. resembles the bacteria Micrococcus spp.
• In what family does Micrococcus spp. belongs?

  Family of: Micrococcaceae
• Biochemical test for Micrococci spp. is:

  • Catalase positive
  • Coagulase negative
• The microscopic examination (morphology) of Micrococcus spp. is:

  • Cocci in pairs
  • Cocci in tetrads
  • Cocci in irregular clusters
• The three other bacteria that share similarities with Staphylococcus spp. and are characterized as gram-positive cocci

  • Rothia mucilaginosa
  • Aerococcus
  • Alloiococcus otitidis
• What color pigment do colonies of Staphylococcus spp. typically produce?

  Yellow pigment
• What is the gram stain reaction of Staphylococcus spp.?

  Gram positive
• Which gram-positive cocci, besides Staphylococcus spp., are part of the normal flora on healthy human skin?

  • Kytococcus spp.
  • Kocuria spp.
• What is the Greek word for "bunches of grapes" from which Staphylococcus was derived?

  Staphle
• Characteristics of Staphylococcus spp.:

  • Non-motile (no flagella)
  • Non-spore-forming bacteria
  • Aerobic
  • Facultative anaerobic
• Staphylococcus spp. is typically found as part of the normal flora on the skin and mucous membranes of humans and animals.
• Staphylococcus colonies are typically medium-sized, around the size of 4 to 8mm
• What are the fastidious strain requirements for Staphylococcus spp.?

  • CO2
  • hemin or;
  • menadione incubated for 48 hours
• When grown in a lab, what colors might scientists observe in colonies of Staphylococcus bacteria?

  • cream-colored (<24 hrs, fresh culture)
  • white or rarely light gold
  • buttery-looking
  • golden yellow (>48 hrs, old culture)
• While most Staphylococcus species are aerobic or facultative, what are the two exceptions that are obligate anaerobes (require no oxygen) and also lack the enzyme catalase (catalase negative)?

  • Staphylococcus saccharolyticus
  • Staphylococcus aureus subsp. anaerobius
• Two bacteria that can develop polysaccharides that appear as a slime layer (gram-positive cocci) and form a biofilm to attach to inorganic surfaces and can overcome antibiotics:

  • Staphylococcus aureus
  • Staphylococcus epidermidis
• What is the expected result for staphylococcus and micrococcus when performing a catalase test?

  Catalase Positive
• The brand name of modified oxidase is Microdase
• What is it called if a bacteria absolutely do not require oxygen to grow, live, and survive?

  Obligate Anaerobe
• What does it called if a bacteria grows, live, and survive in the absence of oxygen

  Anaerobe
• What is the gas requirement for staphylococcus growth?

  aerotolerance: Facultative Anaerobe
• What is the gas requirement for micrococci growth?

  aerotolerance: Obligate Aerobe
• How does staphylococci appear when grown on a specific medium called Furoxone-Tween 80-oil red O agar?

  Negative growth (inhibited by furoxone)
• How does micrococci appear or behave when grown on a specific medium called Furoxone-Tween 80-oil red O agar?

  Positive growth (not inhibited by furoxone)
• What does it called if a bacteria have the ability to grow, line, & survive in small concentration of oxygen environment

  Facultative Anaerobe
• What does it called if a bacteria grows, live, and survive in the presence of oxygen?

  Aerobe
• What does it called if a bacteria requires oxygen to grow, live, and survive?

  Obligate Aerobe
• A bacteria that is resistant (not inhibited) to the antibiotics Bacitracin and Lysosome

  staphylococci
• A bacteria that is susceptible (inhibited) to the antibiotics Bacitracin and Lysosome

  microccoci
• A bacteria that is resistant (not inhibited) to the antibiotic Lysostaphin

  micrococci
• A bacteria that is susceptible (inhibited) to the antibiotic Lysostaphin

  staphylococci
• Staphylococci are commonly NEGATIVE in the Microdase test. However, these four bacteria, which are staphylococci, are POSITIVE in the Microdase test

  • Staphylococcus sciuri
  • Staphylococcus lentus
  • Staphylococcus vitulus
  • Macrococcus caseolyticus
• Micrococci are commonly obligate aerobes. However, these two organisms are facultative anaerobes in terms of aerotolerance:

  • Micrococcus kristinae
  • Micrococcus varians
• A stage of infection caused by S. aures which has no systemic manifestations from the patient

  Folliculitis and Furunucles
• A stage of infection caused by S. aures which has systemic manifestations (such as fever or chills) from the patient

  Carbuncles
• A virulence factor by S. aurues that is serologically grouped by the letters A-E and G-J

  Enterotoxins
• Staphylococcal food poisoning is classified into groups A-E based on the enterotoxins it produce
• Staphylococcal pseudomembranous enterocolitis is classified under letter B of Enterotoxin