More kinetic energy= more successful collisions between the active site and substrate so more enzyme-substrate complexes form.
After the optimum is reached, the enzyme its denatured. The enzyme looses is specific tertiary structure and the active site is no longer complimentary to the substrate - no ESC's form
Factors effecting enzymes - pH
Enzymes denature at extremes of pH. tertiary structure changes shape so the active site is no longer complimentary to the substrate.
H+ ions in acids and O+ ions in alkalines break down hydrogen and ionic bonds.
Enzymes at cold temperatures are inactive not denatured
Factors effecting enzymes - Enzyme Concentration
(Assuming pH/temp is optimum and substrate is unlimited)
More available active sites = more successfulcollisions between the substrate and active site so more enzyme-substrate complexes are formed
More collisions between the active site and substrate = more ESC's form until it plateaus (second green x). All the active sites are occupied so there are no available active sites for collisions.
