STAINING (MICROPARA LAB)

• Refers to the use of colored dyes/ stains to make cells and/or cellular structures visible or to produce contrast between different types of

  cell/cellular components that enhances the contrast of a microscopic image.

  STAINING
• Allows visualization of cells and cellular

  components.

  STAINING
• colored organic compounds(salts) used for staining cells, tissues, microorganisms,etc.

  STAINS
• Stains contain ions which impart them color, these

  ions are called

  CHROMOPHORE
• If chromophore is positive ion, then the dye/stain is called

  BASIC STAIN
• If the the chromophore is negative , then the dye/stain is known

  ACIDIC STAIN
• A molecule which absorbs light at a particular

  wavelength and emits color as a result.

  CHROMOPHORE
• Stains that have negative charge, hence they bind to positively charge

  cellular structures like some proteins.

  ACIDIC STAIN
• stains have a positive charge, hence they bind to negatively charged molecules such as nucleic acids in bacterial walls.

  BASIC STAIN
• Stains are usually formed from precipitation, when aqueous acidic and basic stains are combined

  NEUTRAL STAIN
• 6 STAINING TECHNIQUES IN MICROBIOLOGY ARE AS FOLLOWS:

  • SIMPLE STAINING
  • ACID- FAST STAINING
  • NEGATIVE STAINING
  • ENDOSPORE STAINING
  • GRAM STAINING
  • FLAGELLAR STAINING
• sample of cells or tissue or other material taken from a biological context, spread thinly on a microscope slide for examination.

  SMEAR