Chem Quiz: Chromatography

• Chromatography


  Method to separate substance between a MOBILE and a STATIONARY phase
• Types of chromatography


  • Paper Chro.
  • Gas Chro.
  • Affinity Chro.
  • Flash Chro.
  • Column Chro.
• Paper Chromatography


  A chromatography method which uses chromatography/ filter paper and the substance here are separated on solubility
• How to do paper chromatography

  1. Hang paper over a stick in a beaker

  2. Mark a pencil line on the paper with a pencil dot and the substance in the middle of it

  3. Pour a solvent, at least reaching only under the pencil line

  4. For plant pigment, use 3 drops with a small pause between each drop, 4 leaves, and crush it with the solvent and sand
• Sand


  Break the substance's tissue
• Solvent


  Dissolve chlorophyll
• Solution


  When a solute is dissolved in a solvent. More particles of the solute are dissolved in a concentrated solution than in a dilute solution.
• Solubility


  The maximum amount of solute that can dissolve in 100g of solvent. When a solvent has reached its maximum solubility, which by then it comes to the point where no more of the solid will dissolve, it's called a saturated solution.
• Factors that increase solubility

  • Increasing the amount of solvent
  • Stirring the solvent
  • Increasing the temperature
• Soluble


  Substance that can dissolve
• Insoluble


  Substance that cannot dissolve
• Concentration


  Amount of solute (in g) per volume of solvent (cm3, dm3...)
• Rf Value (Retardation Factor)


  The value used to identify the substance found during chromatography. To search the Rf value found in a chromatography, we must divide the distance of the substance by the distance of the solvent.
• Trivia:The speed of the solvent and the substance is different
• Trivia

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  In a chromatography, the speed of the solvent and the substance is different.