Quantitative Methods

• How do you calibrate a colorimeter?

  Place a blank in. This is a colourless, transparent solution such as distilled water
• What instrument would you use to separate the benedict 's from the reacted precipitate?

  Centrifuge
• A colorimeter is an instrument used to quantitatively measure the transmittance and absorbance of light passing through a liquid sample
• Biosensors use a catalyst to turn a biological response into an electrical signal
• Give an example of a biosensor

  Glucose biosensor
• • Chromatography is a technique that can be used to separate a mixture into its individual components
• What are the two phases of chromatography?

  Mobile phase (solvent)

  Stationary phase (paper)
• Chromatography

  A) pencil

  B) mixture

  C) below

  
• We can use chromatography to figure out what unknown mixtures are composed of
• Rf value = distance moved by solute / distance moved by solvent
• What are the results of chromatography called?

  Chromatogram
• Rf values are specific to the molecule.
• The higher the Rf value, the more soluble the molecule is in the solvent