rp 5: effect of pH on amylase

• p1

  1. place one drop of iodine solution into each well of a spotting tile
  2. 1st test tube: 2 cm3 starch solution
  3. 2nd test tube: 2 cm3 amylase solution
  4. 3rd test tube: 2 cm3 pH5 buffer solution - controls pH
  5. place all test tubes in a water bath at 30 degrees & leave for 10 minutes
• p2

  1. combine all solutions into 1 test tube & stir w stirring rod
  2. return to water bath & start a stopwatch
  3. after 30s, use stirring rod to transfer 1 drop of solution to each spotting tile
  4. repeat step 3 & continue until the iodine remains orange & record time when reaction has finished
  5. repeat whole experiment using different pH buffers
• what will happen when the temperature is too high?

  • enzyme will be denatured
  • starch will not be broken down