producing monoclonal antibodies

• what are monoclonal antibodies?

  antibodies that are clones from one parent cell, they are specific to one type of antigen
• how are monoclonal antibodies produced?

  1. antigen injected into a mouse
  2. mouse lymphocytes are stimulated to produce antibodies
  3. lymphocytes fuse with myeloma cells to form a hybridoma
  4. hybridoma cells are cloned
  5. hybridoma divides rapidly & produces antibody
  6. antibodies are collected