Module 9: Techniques in Microbial Diversity

• CTC stained cells show metabolic activity/active growth
• oxidized CTC stained cells show as colorless and soluble
• reduced CTC stained cells indicate metabolism and show as fluorescent red and insoluble
• FISH: make DNA probe with flourescent tag to bind to denatured sequence
• amplicon sequencing: PCR amplification of 16s rRNA
• metagenomics: PCR amplification of whole genetic sequence
• metabolomics: seperate compounds and use mass spectrometer to identify peaks and make metabolic map
• species have 95-98% identical 16s rRNA