Transmission electron microscope

• Electrons focused using electromagnets
• Electrons pass through specimen, denser parts absorb more and appear darker
• Generates 2D image of a cross-section
• Very high resolution due to short wavelength of electrons
• Can see internal structures of organelles and ribosomes
• Specimen = very thin
• High magnification (x1,000,000)
• Can only view dead/dehydrated specimens as uses a vacuum
• Complex preparation so artefacts often present
• Does not show colour