Fluorescence and FPIa

• FPIA steps

  1. fluorophore attaches to drug we are assaying
  2. wash away antibodies not attached to drug
  3. hit with light energy and measure fluorescence
• fluorophore is an antibody with fluorescent body
• • Fluorescence Polarization
  • Principle - incident light is polarized in a single plane; emitted light will be polarized in the same plane
• fluorescence polarization

  • Energy involved - what excites the photon will be the excitation spectra 
• fluorescence polarization

  • Radiant (emission) energy is polarized in the same single plane as the incident (excitation) light by large molecules
• • FPIA
  • Energy involved - light energy after washing away antibodies not attached