Paper Chromatography

• Chromatography is used to separate a mixture of soluble substances.
• Mobile Phase - the solvent moving through the paper carrying dissolved substances with it.
• Stationary Phase - the surface through which the solvent and dissolved substances move.
• Paper Chromatography:

  • Draw a pencil line close to the bottom of the paper but above the water level
  • Add ink drops onto the pencil line
  • Place in a beaker with water until it reaches the top of the paper
• Substances with a higher solubility will be carried higher up the paper in paper chromatography.
• Rf Value = distance moved by solute / distance moved by solvent