Save
...
3rd Term
Micro
Midterm
Save
Share
Learn
Content
Leaderboard
Share
Learn
Created by
aaaa
Visit profile
Cards (60)
What is Gentian Violet used as in staining?
Primary stain
View source
What type of bacteria retains the violet color in Gram staining?
Gram Positive
View source
What does fixing a smear involve?
Passing the smear over moderate flame
View source
What is a visible group of bacteria on solid medium called?
Colony
View source
What is the substrate used for cultivating microorganisms?
Culture media
View source
What optical instrument is used to study minute living forms?
Microscope
View source
What sterilization apparatus is used for glassware?
Autoclave
,
oven
,
pressure cooker
View source
What does Gram staining classify bacteria based on?
Composition of their
cell walls
View source
What is resolving power in microscopy?
Ability to distinguish two
nearby
points
View source
At what temperature are culture media sterilized for 15 minutes?
121
C
View source
What is the magnification of an objective labeled OIO?
100 X
View source
What plating technique estimates total microbial load?
Pour Plate
View source
Who introduced the first simple microscope?
Anton Van Leuwenhooke
View source
Who introduced the Gram staining technique?
Hans Christian Gram
View source
What can be an ideal substrate for microorganisms?
Fresh
Meat
View source
What is the general solvent used for media preparations?
Distilled water
View source
What are you studying when observing microbial growth on a petri dish?
Cultural
characteristics
View source
What is cultivation of microorganisms in Petri dishes called?
Plating
View source
What does microbiology study?
Minute
organisms invisible to the naked eye
View source
What is a substance used to fix a stain or dye?
Mordant
View source
What is the decolorizer used in Gram staining?
Ethanol
View source
What is the numerical aperture in microscopy?
Optical value stamped on the
lens
View source
What is a mixed culture?
Growth of 2 or more
organisms
together
View source
What is the decolorizer used in acid-fast staining?
Acid alcohol
View source
What organisms resist decolorization in Gram staining?
Gram positive
View source
What is the primary stain used in Gram staining?
Crystal violet
View source
What stain is used in negative staining?
Nigrosin
View source
What part of the microscope adjusts focus?
Coarse and fine adjustment
View source
What part of the microscope reflects light into the base?
Mirror
View source
What part of the microscope supports the head?
Arm
View source
What is the streak plate method?
Inoculum
transferred by streaking across
agar
View source
What is the unit of microbial measurement?
Micron
View source
What is the spread plate method used for?
Enrichment
,
selection
, and
screening
of microbes
View source
What is acid-fast staining used for?
Identifying
Mycobacterium
species
View source
What is selective media used for?
To isolate specific
microorganisms
View source
What is the purpose of transport media?
To maintain viability of
pathogens
View source
What is the primary stain used in simple staining?
Safranin
View source
What is Masson staining used for?
Differentiating cells from
connective tissues
View source
What does staining enhance in biological specimens?
Contrast at the
microscopic
level
View source
What is permeabilization in staining?
Dissolving
cell membrane
for easy staining
View source
See all 60 cards