Midterm

Cards (60)

  • What is Gentian Violet used as in staining?
    Primary stain
  • What type of bacteria retains the violet color in Gram staining?
    Gram Positive
  • What does fixing a smear involve?
    Passing the smear over moderate flame
  • What is a visible group of bacteria on solid medium called?
    Colony
  • What is the substrate used for cultivating microorganisms?
    Culture media
  • What optical instrument is used to study minute living forms?
    Microscope
  • What sterilization apparatus is used for glassware?
    Autoclave, oven, pressure cooker
  • What does Gram staining classify bacteria based on?
    Composition of their cell walls
  • What is resolving power in microscopy?
    Ability to distinguish two nearby points
  • At what temperature are culture media sterilized for 15 minutes?
    121 C
  • What is the magnification of an objective labeled OIO?
    100 X
  • What plating technique estimates total microbial load?
    Pour Plate
  • Who introduced the first simple microscope?
    Anton Van Leuwenhooke
  • Who introduced the Gram staining technique?
    Hans Christian Gram
  • What can be an ideal substrate for microorganisms?
    Fresh Meat
  • What is the general solvent used for media preparations?
    Distilled water
  • What are you studying when observing microbial growth on a petri dish?
    Cultural characteristics
  • What is cultivation of microorganisms in Petri dishes called?
    Plating
  • What does microbiology study?
    Minute organisms invisible to the naked eye
  • What is a substance used to fix a stain or dye?
    Mordant
  • What is the decolorizer used in Gram staining?
    Ethanol
  • What is the numerical aperture in microscopy?
    Optical value stamped on the lens
  • What is a mixed culture?
    Growth of 2 or more organisms together
  • What is the decolorizer used in acid-fast staining?
    Acid alcohol
  • What organisms resist decolorization in Gram staining?
    Gram positive
  • What is the primary stain used in Gram staining?
    Crystal violet
  • What stain is used in negative staining?
    Nigrosin
  • What part of the microscope adjusts focus?
    Coarse and fine adjustment
  • What part of the microscope reflects light into the base?
    Mirror
  • What part of the microscope supports the head?
    Arm
  • What is the streak plate method?
    Inoculum transferred by streaking across agar
  • What is the unit of microbial measurement?
    Micron
  • What is the spread plate method used for?
    Enrichment, selection, and screening of microbes
  • What is acid-fast staining used for?
    Identifying Mycobacterium species
  • What is selective media used for?
    To isolate specific microorganisms
  • What is the purpose of transport media?
    To maintain viability of pathogens
  • What is the primary stain used in simple staining?
    Safranin
  • What is Masson staining used for?
    Differentiating cells from connective tissues
  • What does staining enhance in biological specimens?
    Contrast at the microscopic level
  • What is permeabilization in staining?
    Dissolving cell membrane for easy staining