Microscopy

• What should you be able to do by the end of the video?

  Describe using an optical microscope
• Why is it important to learn the details of using an optical microscope?

  It could come up in a six mark essay
• What is the main part of the optical microscope where the slide is placed?

  The stage
• What is the function of the lamp in an optical microscope?

  To illuminate the microscope slide
• What is used in some optical microscopes instead of a lamp?

  A mirror
• What are the objective lenses used for in an optical microscope?

  To magnify the specimen being viewed
• How many objective lenses do most microscopes have?

  Three
• What are the typical magnifications of the objective lenses?

  Four times, ten times, forty times
• What is the magnification of the eyepiece lens?

  Ten times
• What is the purpose of the coarse focusing dial?

  To adjust the distance between the lens and slide
• What should you do while adjusting the position of the objective lens?

  Look at the microscope from the side
• How do you bring the cells into focus after placing the slide?

  Use the coarse focusing dial
• How do you calculate the total magnification of a microscope?

  Multiply eyepiece magnification by objective magnification
• What is the total magnification when using a ten times eyepiece and a four times objective lens?

  $40 \text{ times}$
• What should you do after selecting a higher power objective lens?

  Adjust the fine focusing dial
• What can you see when looking at animal cells under an optical microscope?

  Nucleus, cytoplasm, and cell membrane
• What can you see when looking at plant cells under an optical microscope?

  Cell wall, cytoplasm, and nucleus
• What should you include in your drawing of cells?

  A magnification scale
• How do you create a magnification scale for your drawing?

  Measure the diameter of the field of view
• What should you write on your drawing besides the scale?

  The magnification used
• What is the purpose of the optical microscope?

  To view cells on a prepared slide
• What is a key limitation of using an optical microscope?

  Limited detail of cellular structures
• How does the view of animal cells differ from plant cells under a microscope?

  Animal cells lack cell walls and chloroplasts
• What is the function of the clips on the stage of the microscope?

  To hold the slide in place
• What is the role of the light source in microscopy?

  To provide illumination for viewing specimens
• What is the significance of the eyepiece lens in magnification?

  It contributes to the total magnification
• How do you make a labeled drawing of cells?

  Use a pencil to draw and label
• What types of details are visible in animal cells under a microscope?

  Nucleus, cytoplasm, and cell membrane
• What types of details are visible in plant cells under a microscope?

  Cell wall, cytoplasm, and nucleus
• How do you measure the field of view?

  Using a clear plastic ruler
• Why is it important to use a pencil for drawing?

  Pencil allows for easy corrections
• What potential exam questions relate to the practical?

  Questions on using an optical microscope
• What is the significance of the coarse and fine focusing dials?

  They adjust the focus of the microscope
• How do animal and plant cells differ in visibility under a microscope?

  Animal cells lack cell walls and chloroplasts