1. Label each well with the time (from 0 onwards)
2. Add a drop of iodine solution to each well
3. Add 2 cm' of each buffer solution using a syringe (ranging from pH 3.0 to 7.0) into each labelled test tube
4. Immerse the starch solution, amylase solution, and the test tubes of buffer solution in a water bath at 25°C
5. Allow a few minutes for the temperature to equilibrate
6. Use a syringe to add 2 cm' of amylase into a test tube of buffer solution
7. Use a syringe to add 2 cm' of starch into the same test tube and start timing immediately
8. Use the glass rod to transfer a drop of the mixture to the well labelled 'O' on the tile
9. Repeat step 6 every 30 seconds, rinsing the glass rod in between every test, until the iodine solution remains brown and does not turn blue-black
10. Calculate the rate of enzyme reaction by using 1/ time taken for iodine solution to remain brown
11. Repeat steps 2-8 for buffer solutions with different pH values
12. Plot a graph of the rate of enzyme reaction against pH
