🫀MIDTERM HPCT trimming and sectioning

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Created by
Ushtine Q

Cards (60)

  • TRIMMING
    ​
    o Process of removing excess wax after embedding.
    o Excess wax is cut off from the block to expose the
    tissue surface in preparation for actual cutting.
  • FOUR-SIDED PRISM/TRUNCATED PYRAMID

    Ideal knife or blade for trimming
  • SECTIONING

    The process by which a processed tissue is cut into uniformly thin
    slices (sections) to facilitate studies under the microscope
  • (CUTTING OR MICROTOMY

    other name for SECTIONING
  • 4-6 um

    thickness for ROUTINE HISTOLOGIC PROCEDURE.
  • 10-15 um

    measurement for sectioning FROZEN SECTION
  • 0.5 um

    measurement for sectioning for ELECTRON MICROSCOPY.
  • PLANE-CONCAVE KNIFE

    One side of the knife is flat while the other is concave.
    ​
  • 25 mm in length.

    length PLANE-CONCAVE KNIFE
  • 120 mm in length
    length BICONCAVE KNIFE
  • BICONCAVE KNIFE

    With both sides
    concave, recommended for cutting paraffin embedded
    sections on a rotary microtome
  • 100 mm in length

    length PLANE-WEDGE KNIFE
  • PLANE-WEDGE KNIFE

    Have both
    sides straight, recommended for frozen sections or for
    cutting extremely hard and tough specimens
    embedded in paraffin blocks, using a base sledge type
    or sliding microtome
  • Less concave sides

    concave recommended for cutting celloidin embedded
    tissue blocks on a sliding microtome.
  • More concave sides

    concave used to cut paraffin sections on base
    sledge, rotary or rocking microtome
  • BEVEL

    cutting facet, found on the tapered edge of all knives,
    the sides of which are more acutely inclined towards each other
    ​
  • BEVEL ANGLE

    the angle formed between the cutting edges.
    About 27Ëš to 32Ëš.
  • 27Ëš to 32˚

    BEVEL ANGLE
  • CUTTING ANGLE

    sides of the knife are inclined at 15Ëš angle
    maximum penetration of the tissues and minimize distortion
  • 15Ëš angle

    CUTTING ANGLE
  • CLEARANCE ANGLE

    the knife should be inclined with a 5-10Ëš
    angle will prevent uneven sections and will prevent the cutting
    facet to compress the tissue block during the cutting process
  • 5-10Ëš
    angle
    CLEARANCE ANGLE
  • "ralph knives'' / Glass knives

    knives used for for ultramicrotomy
  • Diamond knives

    knives used for cutting resin section
  • o Disposable knives
    o Stainless steel
    o Tungsten carbide
    o Glass knives = "ralph knives'' for
    ultramicrotomy
    o Diamond knives = cutting resin section
    o Steel knives

    KNIFE MATERIALS
  • HONNING (HARD SHARPENING)

    Removal of nicks (irregularities)
  • HONNING (HARD SHARPENING)

    HEEL TO TOE 20-30 TIMES
  • Mechanical honing

    honing: makes use of a vibrating frosted plate or wheel driven by an electrical motor.
  • HONING PROPER

    removal of blemishes and grinding the
    cutting edge of the knife on a stone to acquire an even edge.
  • XYLENE

    The surface of the hone is wiped clean with a soft cloth
    moistened with __ in order to remove the scattered small
    particles of stones and metal.
  • 10-20

    For plane wedge knife: The knife is turned over to sharpen the other surface every __-__ strokes
  • surface

    For plane concave knives: Only the __ should be
    rubbed on the hone
  • ¼ to 3/8, 14, 1-2

    honing: ADDITIONAL: use a circular glass plate usually __-__ inch thick, about __ inches long and __-__ inches wider than the length of the knife with finely powdered aluminum oxide made into paste with water is used as an abrasive (polishing)
    ​
  • Belgium yellow

    hone: for manual sharpening when cutting edge has been rendered blunt or nicked. This type usually gives the BEST RESULT
  • Arkansas

    hone: Gives more polishing effect than the belgium yellow
  • Fine carborundum

    hone: much coarser than the first two typed and
    used only for badly nicked knives. Followed by either one of the first 2 knife sharpeners
  • 8x3

    hone should be long enough size: __
  • STROPPING
    ​
    o To polish and sharpen the cutting edge
    o Removal of burrs
    ​
  • Xylene

    After honing, the knife is wiped with ___ to remove oil or soap
    and then stropping follows
    ​
  • STROPPING

    From toe to heel direction