MICROPARA LAB BASICS OF MICROORGANISM IDENTIFICATION

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Created by
Kim

Cards (46)

    1. specific bacterium with large rod?
    a=fusobacterium
  • 2. difference between spirillum and spirochetes?
    a= spirillum is rigid, spirochetes are flexible
  • 3. microorganism with corkscrew form?
    a= Borellia burgdorferi
  • 4. grape-liked clusters?
    a= staphylococci
  • 5. chains arrangement?
    a= streptococci
  • 6. smallest pathogenic bacillus?
    a= Haemophilus ( 0.2x 0.5 um)
  • 7. largest pathogenic bacillus?
    a= Bacillus anthracis (1x 3-10 um) agent for bioterrorism
  • 8. capsulated capsule ?SKHPNC
    A= Streptococcus pneumoniae, Klebsiella pbeumoniae, Haemophilus influenzae, Pseudomonas aeruginosa , Neisseria menigitidis, Cryptococcus neoformans
  • 9. the only capsulated fungi?
    a= Cryptococcus neoformans
  • 10. negative staining in capsule?
    a= India ink stain
  • 11. cell wall is also known as?
    a= peptidoglycan
  • 12. functions of cell wall?
    a= protects bacteria, Gives shape, protects from antimicrobial agents
  • 13. Components of cell wall? ex 4
    a= Lipoteichoic acid, Lipopolysaccharide, periplasm, cytoplasmic
  • 14. result of lipopolysaccharide in Gram negative?
    a= high
  • 15. in microscopy for viruses we can use?
    a= Fluorescence microscope, electron microscope
  • 16. most common type oof microscope?
    a= bright field microscope
  • 17. cmmon and mos used technique to diffrentiate gram negative and positive bacteria?
    a= gram staining technique
  • 18. gram staining technique was discovered by?
    a= Hans Christian Gram 1882
  • 19. reagent for primary stain in gramm staining technique?
    a= crystal violet
  • 20. secondary stain reagent?
    a= safranin
  • 21. hot method?
    a= ziehl neelsen method
  • 22. cold method?
    a= kinyoun method
  • 23. in cold method we uses reagent?
    a= tergitol/ phenol'
  • 24. example of acid fast bacteria?
    a= mycobacterium and nocardia
  • 25. acid fast staining is usually us for?
    a= sputum testing
  • 26. bacteria with mycolic acid ex?
    a= Mycobacterium, Nocardia, Corynebacterium, Rhodococcus
  • 27. the positive result for Auramine rhodamine stain?
    a= mycobacteria appears as slender rod that fluorescence bright yellow
  • 28. if auramine O is used positive result? combination of auramine and rhodamine stain?
    a= Bright yellow, yellow red
  • 29. what microscope is used in Auramine and rhodamine?a=Fluorescence microscope
  • 30. test used for spore formers?
    a= Schauffer- Fulton
  • 31. 2 ex of bacteria that are spore forming?
    a= Bacillus / Clostridium
  • 32. in flagellar staining method it used for PRIOR staining?
    a= potassium alum, tannic acid
  • 33. process of putting organism in slide?
    a= smear
  • 34. in smear it used for labeling?
    a= frosted edge
  • 35. in culture dish this is where the bacteria grows?
    a= culture media
  • 36. complex polysaccharide derived from algae commonly used in culture media?
    a= agar
  • 37. in culture media give the example of synthetic?
    a= Czapek Dox medium
  • 38. chemically defined media and is produced from pure chemical substance?
    a= synthetic
  • 39. it is used to increase very small number to detectable levels?
    a= blood, serum, egg yolk
  • 40. agar for anaerobic/ reducing?
    a= thioglycolate agar