investigating effect of pH on enzyme activity
1) Put a drop of iodine solution into every well of the spotting tile.
2) Place a Bunsen burner on a heat-proof mat and a tripod and gauze over until it is 35C use a warm bath to keep temp constant
3) Use a syringe to the 1cm3 of amylase solution and 1cm3 of a buffer solution with a pH of 5 to a boiling tube.
4) Next, use a different syringe to add 5cm3 of a starch solution to the boiling tube
5) Immediately mix the contents of the boiling tube and start a stop clock
6) Use continuous sampling to record how long it takes for the amylase
7) When the iodine solution goes brown/orange, starch is no longer present.