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Biology research design and analysis
exam 2
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Cards (62)
what are the 3 sources of variation
random
error
variation
treatment
effects
experimental artifacts
sample vs tails
sample-
number of
groups
tails
- side of
distribution
can be
1
or
2
what to do if one of the assumptions of normally parametric tests are violated?
first test for normality
if tests arent normal,
transform
data
:
log
square root
what is
psuedoreplication
?
data
that is not
independent
artificially inflates sample size
Tranformations
of
Data
alter
data scale
improve
normality
log
transformations
squareroot
transformation
F-Test
Post Hoc Test
Never run a post hoc unless
anova
shows a
significant
P-value
Steps
for
post
hoc
analysis
Order
means
largest
to
smallest
Determine
Psnk
Calculate
Sab
calculate
q
DF-
form
anova table
Find
q crib
Determine
significance
Sab equal sample size
√
(
m
s
e
r
r
o
r
/
n
)
√(mserror/n)
√
(
m
serror
/
n
)
Sab for an
unequal sample size
√
(
m
s
e
r
r
o
r
/
2
)
(
1
/
n
a
+
1
/
n
b
)
√(mserror/2)(1/na+1/nb)
√
(
m
serror
/2
)
(
1/
na
+
1/
nb
)
equation
for
q
post hoc equation
q=(Xa-xb)/sab
Statistical Inference
makes an estimate, prediction, or decision about a population based on a sample
ex: hypothesis testing
Descriptive Statistics
organize
,
summarize
and
present
data
usually
graphical
or
numerical
ex:
frequency distribution
,
central tendency
,
variability
of
data set
Presenting Data
never report raw
present
summaries
of
data
-
descriptive statistics
Tables
and
FIgures
Table
- arranges data in
rows
and
colomns
Figure-
graphical representation
How does the
width
of the
interval
change as
alpha changes
?
as
alpha decreases
,
interval
gets
larger
(table 2.)
Confidence Interval
interval values
computed from the sample
table
2
width
of the
interval
chages
as
alpha
changes
as
alpha
gets
smaller
interval
gets
larger
How to Interpret 95% confidence interval
we are
95% confident
that the
true population mean
is
between these values
Parametric Statistics
Assuptions
data
are
normally distributed
Data
are
independent
variances
are
equal
Factors that confidence interval depends on
Sample mean
Level
of
confidence
SE
Scientific
Hypothesis
Testable
and
falsifiable
F-Test
compares
2 sample variances
F=
Sa^2/Sb^2
equal variance F=
1
One sample T-Test
compares sample mean
(
x
) to
proposed population mean
(u)
tdf
=
X-u
/
se
T-Test
only compares
2 groups
looks at
means
Paired sample T-Test equation
tdf
=
d-u/se
H0- no difference
u=0
d= avg difference
Measurements
of
Distribution
Central Tendency
Dispersion
Changing mean
(
u
)
shifts distribution left
or
right
changing Sd
(s)
increases
or
decreases
the
spread
Common test for
normality
H0
=
distribution
is
normal
,
no effect
shapiro wilk
Anderson- Darling
Kolmogrov- Smirnov
Lillefors
Features
of a
good Research Design
random variation
estimation
of
treatment effects
High degree
of
accuracy
and
precision
Absence
of
bias
Simplicity
in
execution
and
analysis
Presenting Tables
Properly labeled
data organized
within a row
descriptive caption above table
includes AVG
,
SE
, n
Inferential Statistics
analysis
and
interpretation
of
data
to make general conclusions and inferences
draws conclusions and makes predictions about population data
Descriptive Statistics
summarization of data
displayed in tables and Figures
How to minimize Random Error Variation and Experimental Artifacts?
high degree of
precision
and
accuracy
effective controls
Abscence of bias
Experimental Artifacts
variation
due to factors other than the
experimental treatment
or
condition
Random Error Variation
results from
influences
of
independent
events
existing variation
- gentotypic variation
measurement
error
random chance
events
Presenting Figures
graphical
X-axis has
IV
Y axis has
DV
labeled axis
Inlcudes units
no
title
descriptive captions
below
Treatment Effects
variation
that is a result of your treatments
Experimental Differences
Anova Comparision
compares population
variance
Factor
/
Error
factor- based on means
error
-
based
on
individuls
F=1
Sources
of
Variation
Random
error
variation
treatment
effects
experimental artifacts
Statistical Hypothesis
Statments
about whether or not a
pattern
or
trend
or
difference
is
present
in
data
simply statements about whether or not a
pattern
or
trend
exists
Paired sample t-test equation
tdf=d-u/se
Confidence Interval
equation
x+
,
-
(
tcrit
)(
SE
)
or
u-Tvalue*SE
<
x
<u+
Tvalue*SE
Confidence Interval Depends on
Sample mean
(x)
SE
level
of
confidence
(95%)
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