Transmission electron microscope (TEM)

• • use electromagnets to focus a beam of electrons, which is then transmitted through the specimen to produce 2D images
  • Denser parts of the specimen absorb more electrons, which makes them look darker on the image you end up with
• • provide high resolution images, so they can be used to look at very small organelles
  • they can also be used to look at the internal structures of organelles in detail
• • But specimens viewed on TEMs need to be quite thinly sliced
  • The angle at which specimens are cut can affect how they appear